A molecular staging model for accurately dating the endometrial biopsy

WT Teh; J Chung; SJ Holdsworth-Carson; JF Donoghue; M Healey; HC Rees; S Bittinger; V Obers; C Sloggett; R Kendarsari; JN Fung; S Mortlock; GW Montgomery; JE Girling; PAW Rogers

Journal article

A molecular staging model for accurately dating the endometrial biopsy

WT Teh, J Chung, SJ Holdsworth-Carson, JF Donoghue, M Healey, HC Rees, S Bittinger, V Obers, C Sloggett, R Kendarsari, JN Fung, S Mortlock, GW Montgomery, JE Girling, PAW Rogers

Nature Communications | NATURE PORTFOLIO | Published : 2023

DOI: 10.1038/s41467-023-41979-z

Abstract

Natural variability in menstrual cycle length, coupled with rapid changes in endometrial gene expression, makes it difficult to accurately define and compare different stages of the endometrial cycle. Here we develop and validate a method for precisely determining endometrial cycle stage based on global gene expression. Our ‘molecular staging model’ reveals significant and remarkably synchronised daily changes in expression for over 3400 endometrial genes throughout the cycle, with the most dramatic changes occurring during the secretory phase. Our study significantly extends existing data on the endometrial transcriptome, and for the first time enables identification of differentially expre..

View full abstract

University of Melbourne Researchers

Wan Teh Author

Jessica Chung Author

Sarah Holdsworth-Carson Author

Jacqueline Donoghue Author

Related Projects (2)

UTERINE CONTROL OF EMBRYO IMPLANTATION

Successful establishment of pregnancy requires a viable embryo and a receptive uterus. The contribution that reduced uterine receptivity mak..

IDENTIFICATION AND FUNCTION OF GENES THAT INCREASE RISK FOR ENDOMETRIOSIS

Endometriosis is a common disease that affects 7% of reproductive age women in Australia, resulting in pelvic pain, subfertility and painful..

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

We acknowledge the work of Research Nurses Ranita Charitra, Tracy Middleton and Irene Bell who recruited all the participants in this study and Leonie Cann who provided laboratory support. Thank you to the surgeons and theatre booking staff at the Royal Women's Hospital and Staff at Melbourne IVF. We also acknowledge and thank the many women who provided tissues and clinical data that were used in this study. Research reported in this publication was supported in part by National Health and Medical Research Council (NHMRC) project grants GNT1012245 and GNT1105321 (PAWR, GWM, JEG, SJH), NHMRC Fellowship GNT1078399 and Investigators Grant GNT1177194 (GWM), NHMRC Medical Postgraduate Scholarship No. 1055814 and RANZCOG Fellows' Clinical Research Scholarship (2013) (WTT), funds from Melbourne IVF, and the Medical Research Future Fund MRF1199715 (PAWR, MH, SJH, JFD).